Tracking of Fluorescent Cells Based on the Wavelet Otsu Model

Abstract

The mainstay of the project is to demonstrate that the proposed tracking scheme is more accurate and significantly faster than the other state-of-the-art tracking by model evolution approaches.The model is validated by comparing it to the original algorithm.The proposed tracking scheme involves two steps. First, coherence-enhancing diffusion filtering is applied on each frame to reduce the amount of noise and enhance flow-like structures. Second, the image segmentation is done by the Wavelet OTSU method in the fast level set-like and graph cut frameworks. This model evolution approach has also been extended to deal with many cells concurrently. The potential of the proposed tracking scheme and the advantages and disadvantages of both frameworks are demonstrated on 2-D and 3-D time-lapse series of mouse carcinoma cells.